LIMBO

Light Microscopy Bioimaging Operations Consortium

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Microscopy Imaging

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Live cell imaging

Ultrafast (kHz) imaging

Electrophysiology&imaging

Multiphoton

High-content imaging

Molecular dynamics

CLEM/cryo-EM

Lightsheet

Second harmonic imaging

Super resolution

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Multiphoton

Specialties and expertise:

 

LIMBO is renowned for dynamic tissue imaging, including the following topics: 

  • Biology: Functional and quantitative imaging of central (brain) and peripheral (lung and gut) nervous system: 

    • Deep tissue imaging 

      • Whole brain clearing and imaging

      • 2-Photon

    • Live, in vivo and ex vivo imaging 

      • Live cell imaging of lung slices and gut preparations

      • Calcium imaging and electrophysiology

      • Functional analysis of primary neuronal networks

      • Morphological analysis 

    • Nanomaterial interactions and tracking with biological tissues and cells

  • Technology:

    • Microscopy development (super-resolution, compact microscopy for space and microgravity, low photo toxicity modes)

    • High content workflows and top-down cytotoxicity assays: viability, DNA damage, oxidative stress, mitochondrial dysfunction (towards e.g. nanoparticles, meat digests, plant toxins)

    • Dynamic molecule imaging (FCS, FRAP)

 

Offered Technologies

  • Deconvolution widefield microscopy

  • Laser scanning confocal microscopy (LSCM)

  • Electron microscopy

  • Multiphoton systems

  • Spinning disc confocal systems

  • Total internal reflection fluorescence microscopy (TIRF)

  • Correlative light electron microscopy (CLEM)

  • Fluorescence correlation spectroscopy (FCS)

  • Fluorescence recovery after photobleaching (FRAP)

  • Fluorescence resonance energy transfer (FRET)

  • Raman Spectroscopy

  • High Throughput Microscopy

  • Photo activated localization microscopy (PALM)

  • Stochastic optical reconstruction microscopy (STORM)

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FLAMINGO

Flanders Molecular Imaging Organisation

Biomedical Imaging

Human (clinical) imaging

Large animal imaging

Small animal imaging

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Hardware development

Image reconstruction

Kinetic modelling

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Biomedical Probe Development

PET (GMP)

SPECT

Therapeutic probes

Contrast agents, optical

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Specialties and expertise:

Multimodal imaging, translational imaging, clinical trials, full GMP radiopharmaceutical development, kinetic modelling and multimodal data analysis.


​Offered Technologies

  • ​MRI-PET

  • microCT

  • (micro)PET/CT

  • microMRI/MRS

  • microSPECT/CT

  • microUS

  • Optical Imaging



Additional services offered:

  • Methodological setup (e.g. design of study protocol and standard operation procedures)

  • Technical assistance to run instruments and specialised technologists

  • Fully equipped animal facilities, including monkeys

  • Full-range data processing and analysis

  • Preclinical and clinical expertise in academic and industry-sponsored trials

  • Training rooms

  • Seminar rooms

  • Extensive GMP-radiopharmaceutical development (18F, 68Ga, 11C, 13N, 15O)

  • Nanobody platform for radio-immune-imaging

Technology

The institutions that make up Flanders BioImaging have in-depth expertise across the whole imaging range, with each site having 'spearpoint' expertise for specific techniques and applications. By intergrating operations, platform techniques are available for researchers in Flanders and beyond.

Contact

Christopher Cawthorne
Flanders BioImaging Co-ordinator

O&N I Herestraat 49 - box 505
3000 Leuven
Belgium
room: 05.616

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Tel: 0032-16-325694

christopher.cawthorne@kuleuven.be

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