Specialties and expertise:

 

LIMBO is renowned for dynamic tissue imaging, including the following topics: 

• Biology: Functional and quantitative imaging of central (brain) and peripheral (lung and gut) nervous system: 

  • Deep tissue imaging 

    • Whole brain clearing and imaging

    • 2-Photon

  • Live, in vivo and ex vivo imaging 

    • Live cell imaging of lung slices and gut preparations

    • Calcium imaging and electrophysiology

    • Functional analysis of primary neuronal networks

    • Morphological analysis 

  • Nanomaterial interactions and tracking with biological tissues and cells

• Technology:

  • Microscopy development (super-resolution, compact microscopy for space and microgravity, low photo toxicity modes)

  • High content workflows and top-down cytotoxicity assays: viability, DNA damage, oxidative stress, mitochondrial dysfunction (towards e.g. nanoparticles, meat digests, plant toxins)

  • Dynamic molecule imaging (FCS, FRAP)

 

Offered Technologies

• Deconvolution widefield microscopy

• Laser scanning confocal microscopy (LSCM)

• Electron microscopy

• Multiphoton systems

• Spinning disc confocal systems

• Total internal reflection fluorescence microscopy (TIRF)

• Correlative light electron microscopy (CLEM)

• Fluorescence correlation spectroscopy (FCS)

• Fluorescence recovery after photobleaching (FRAP)

• Fluorescence resonance energy transfer (FRET)

• Raman Spectroscopy

• High Throughput Microscopy

• Photo activated localization microscopy (PALM)

• Stochastic optical reconstruction microscopy (STORM)

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