Fast and directly correlated integration of light and electron microscopy

We developed a targeted approach that allows to image specific tissue features, like organelles, cell processes, and nuclei at different scales to enable fast, directly correlated in situ array tomography using an integrated light and electron microscope (iLEM-AT).

Our method facilitates tracing and reconstructing cellular structures over multiple sections, is targeted at high resolution ILEMs, and can be integrated into existing devices, both commercial and custom-built systems.

Read more:

Gabarre, S., Vernaillen, F., Baatsen, P., Vints, K., Cawthorne, C., Boeynaems, S., Michiels, E., Vandael, D., Gounko, N. V., & Munck, S. (2021). A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography. BMC biology, 19(1), 152. https://doi.org/10.1186/s12915-021-01072-7